Suggested use is for developing fingerprint residue (Amino Acids) on paper and porous items including raw wood and some currencies. 1,2 Indanedione has a high florescence when excited by a Laser or ALS in the 475-590nm range giving greater clarity and background elimination.

Saturate evidence in 1,2 Indanedione solution; allow the item to air dry inside the fund hood. Latent prints may develop within a few hours or may take a few days at room temperature. Greater development may be achieved by sequencing with subsequent applications of DFO and Ninhydrin. To speed up the development process, apply humid heat. This is best achieved in a humidity chamber; Ideal settings are 212°F at 80% humidity for 15-20 minutes. A steam iron may be used being cautious not to touch the paper or item directly with the iron as it can scorch the item destroying the evidence.
The only acceptable carrier and formulation is with 3M™ HFE7100. We have found 3M™ HFE7100 to be the best overall solution for penetration, ease of use, user and environmental exposure, rapid drying times, and quality/quantity of developed latents.


- 1,2 Indanedione
- Ethyl acetate
- 3M™ HFE7100
- Scale, graduated cylinders, beakers, trays, magnetic stirrer and stirring bar, amber storage bottles
- Forensic laser or light source
- Laboratory oven, photographic-mounting press

Safety Considerations

The following safety precautions were adopted:

- Those stated in the chemical manufacturer’s literature
- Use of forensic light source protective goggles
- Use of fume hood
- Use of suitable protective clothing, gloves, and goggles
- Use of precautions to prevent contact with skin and eyes

Process for Premix

1. Saturate the Item to be processed by submerging or soaking with a float bottle application. Spraying 1,2 Indanedione exposes the user to unnecessary risk and does not saturate as thoroughly.
2. Allow item(s) to air dry within a vented fume hood.
3. Latent prints will begin to develop as soon as 30 minutes after treatment or may take as long as 48 hours. The exposure to heat and humidity will usually accelerate the process but prints have developed 24 hours or more subsequent to the heat and humidity acceleration. As a general rule always allow your evidence 24 hours post treatment for a complete exam.
4. The 1,2 Indanedione developed prints will give high fluoresce between 475nm and 590nm.

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Process when using Powder

1. Add 2.0 g of 1,2 Indanedione to 70 mL of ethyl acetate and place on a stirring device for approximately 20 minutes until the IND is dissolved.
2. Add the dissolved mixture to 930 mL of 3M™ HFE7100 solvent.
3. Place the appropriate safety label and information on the bottle. Proper labeling should include:
- Name of Reagent
- Date of Preparation
- Date of Expiration (if applicable)
- Preparer’s name and initials
-  Batch Number
4. 1,2 Indanedione may be applied by dipping or spraying.
5. Items that have been processed with IND may be placed in a humidity chamber at approximately 80°C to 100°C and 60% to 80% humidity for 10 to 20 minutes to accelerate the development of latent prints.
NOTE: Do not use heat on specialty papers (i.e. thermal) because it will darken the paper.
6. Developed latent prints will fluoresce under a green LASER/ALS and are viewed with orange goggles.

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Additional Equipment for Improved Identifications
1. Use of the FF-1.0 Forensic Filter for photography

2. Use of the Dual77 or TracER Laser for excitation

3. Use of the FSIS for live image preview and capture for AFIS submission


Download this Sample SOP guideline for introducing 1,2 Indanedione into your sequencing